Dental pulp stem cell processing

As one of the world’s largest family stem cell banks, we first developed an industry-standard dental pulp processing method in 2011.

For us, it’s important that each client understands the process of preserving and storing their child’s dental pulp stem cells, for complete transparency and peace of mind. Our processing method ensures the highest possible cell count for every sample for effective future treatment opportunities.

Dental pulp processing method

Here we explain the processing method of every tooth sample at Future Health Biobank and how we prepare your sample for storage.

What does each tooth contain?

Teeth, particularly milk teeth, contain a valuable source of stem cells. These are found within the dental pulp at the centre of each tooth and are known as mesenchymal stem cells (MSCs), similar to those found in umbilical cord tissue. At present, MSCs are being investigated in over 1,000 clinical trials to treat a range of diseases such as arthritis, type 1 diabetes and multiple sclerosis (MS).

Here at Future Health Biobank, one milk tooth usually contains enough dental pulp MSCs for successful processing and storage, should they ever be needed in future medical treatments.

Dental pulp processing method

Since the introduction of our dental pulp stem cell banking service in 2011, the scientists at Future Health Biobank have been constantly refining and improving our processing method; the cryopreservation of cultured cells.

On arrival at our laboratory, the dental pulp sample is transferred into a GMP grade? Good Manufacturing Practice. GMP guidelines specify the purity of the air in cleanrooms, ensuring a sterile environment for processing samples. cleanroom. In the clean room, the dental pulp is extracted from the tooth and digested with a combination of enzymes?Enzymes are naturally occurring proteins, used to gently break down the tissue in order to release the cells for culture. to release the cells from the pulp.

Once the cells have been extracted from the tissue, they are transferred into a sealed culture flask containing a growth medium,? We use a growth medium specifically formulated for cell culture. This contains all the nutrients the cells require to grow and multiply. then incubated at 37°C to allow the cells to grow.

Once the cells have grown to the desired level, they are removed from the flask, counted, and transferred to a number of cryovials and a cryoprotectant?A chemical which protects the cells from damage during the freezing process. solution containing DMSO (Dimethylsulphoxide)?Provides high levels of protection to the cells during freezing and over long period of cryogenic storage. is added. The sample is then cooled to -150°C using a programmed controlled-rate freezer. Lastly, the samples are transferred into long-term storage below -160°C in vapour phase liquid nitrogen.